Composite

Part:BBa_K1890011

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-10-07)
Revision as of 10:44, 8 October 2016 by Lycka (Talk | contribs)


mVenus with strong consitutive promoter and RBS

Indroduction

mVenus is a variant of yellow fluorescent protein (YFP) which contains several mutations that make it more stable and provide an improved speed and efficiency of maturation [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and RBS BBa_B0030.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87–90 (2002).


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