Composite

Part:BBa_K1890010

Designed by: Lycka Kamoen, Maria Vazquez   Group: iGEM16_TU_Delft   (2016-10-07)
Revision as of 15:21, 7 October 2016 by Lycka (Talk | contribs)


mCerulean with strong consitutive promoter and RBS

Indroduction

mCerulean is a cyan fluorescent protein derived from ECFP. It presents a series of mutations that increase its extinction coefficient, quantum yield and fluorescence lifetime [1]. We expressed it under the control of the strong constitutive promoter BBa_J23100 and strong RBS BBa_B0030.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Rizzo, M. a, Springer, G. H., Granada, B. & Piston, D. W. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445–449 (2004).


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