Coding
BD-CIB1

Part:BBa_K1884001:Design

Designed by: Chengrong Xie   Group: iGEM16_SZU-China   (2016-10-07)
Revision as of 08:43, 7 October 2016 by Xiechengrong (Talk | contribs) (→‎References)


Gal4BD-CIB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


Design Notes

Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1. They interact in a blue light-specific manner in yeast and Arabidopsis cells, acting together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. The modules require no exogenous chromophore, trigger protein interactions on a subsecond time scale that are reversible within minutes and can be activated by two-photon microscopy, allowing potential use in vivo in whole organisms.


Source

GalBD+CIB1

References

Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.