Part:BBa_K1884001:Design

Gal4BD-CIB1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 218
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 637
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 137
  

Design Notes

Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1. They interact in a blue light-specific manner in yeast and Arabidopsis cells, acting together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. The modules require no exogenous chromophore, trigger protein interactions on a subsecond time scale that are reversible within minutes and can be activated by two-photon microscopy, allowing potential use in vivo in whole organisms.

Source

We gain this part from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.

References

Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.