Plasmid_Backbone
Part:pSB1AC3
Designed by: Tom Knight Group: iGEM05_Registry_Example (2005-06-07)
Revision as of 02:49, 9 June 2007 by Smelissali (Talk | contribs)
High copy BioBrick assembly plasmid
pSB1AC3 is a high copy number plasmid carrying ampicillin and chloramphenicol resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). For the version of this plasmid with a CcdB insert, see BBa_P1010.
Annotated APE file for pSB1AC3
Usage and Biology
Chloramphenicol resistant up to 3500 ug/ml. Tetracycline sensitive at 1.5 ug/ml. Kanamycin sensitive at 5 ug/ml (spayne and tk 6/6/05)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3040 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3034
Illegal XhoI site found at 1035
Illegal XhoI site found at 1927 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3034
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3034
Plasmid lacks a suffix.
Illegal XbaI site found at 3049
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2073
[edit]
Categories
Parameters
//plasmidbackbone/assembly
//plasmidbackbone/copynumber/high
//plasmidbackbone/copynumber/high
chassis | |
copies | 100-300 |
insert | None |
mcs | BioBrick |
origin | pMB1 |
resistance | AC |