Protein_Domain
Part:BBa_K2017000
Designed by: Monica Victoria Gutierrez Salazar Group: iGEM16_Valencia_UPV (2016-10-03)
C-split Cas9 + DnaE C-intein
Half of the SpCas9 protein fused to DnaE C-intein. The active full-lenght spCas9 can be recovered by using the other half of the protein, N-split Cas9 + DnaE N-intein. Inteins are used to fuse both parts of the protein, as they interact and splice, leaving the active protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2020
Illegal PstI site found at 409
Illegal PstI site found at 643
Illegal PstI site found at 1855
Illegal PstI site found at 2159 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2020
Illegal PstI site found at 409
Illegal PstI site found at 643
Illegal PstI site found at 1855
Illegal PstI site found at 2159 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2020
Illegal BamHI site found at 203
Illegal XhoI site found at 1741 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2020
Illegal PstI site found at 409
Illegal PstI site found at 643
Illegal PstI site found at 1855
Illegal PstI site found at 2159 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2020
Illegal PstI site found at 409
Illegal PstI site found at 643
Illegal PstI site found at 1855
Illegal PstI site found at 2159
Illegal NgoMIV site found at 937 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/nbenthamiana
//function/crispr
//function/crispr
None |