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Part:BBa_J100255

Designed by: Helen Webster   Group: Campbell M Lab   (2016-04-12)
Revision as of 15:22, 8 July 2016 by Macampbell (Talk | contribs)

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tCloneTet+PDB riboswitch

The PDB riboswitch consists of a theophylline aptamer sequence from the Protein Databank. This aptamer sequence was inserted into the Wachsmuth et al. RS3 riboswitch sequence using Golden Gate Assembly. The tCloneTet plasmid backbone was obtained from part J119374, and Golden Gate Assembly was used to ligate the riboswitch into the tCloneTet plasmid.

Note: This part has been improved. See Part:BBa_J100279

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 497
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1051
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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