Composite

Part:BBa_K1783002:Design

Designed by: Chun Mun Loke, Adam Wahab, Kimia Abtahi, Iowis Zhu   Group: iGEM15_UMaryland   (2015-07-18)
Revision as of 04:35, 26 September 2015 by Kabtahi (Talk | contribs)

Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. The BioBrick was transformed into both E.coli K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.

Source

Promoter, RBS, LVA tag from E. coli RFP from Discosoma striata

References