Composite

Part:BBa_K1783002:Design

Designed by: Chun Mun Loke, Adam Wahab, Kimia Abtahi, Iowis Zhu   Group: iGEM15_UMaryland   (2015-07-18)

Constitutive Promoter-RBS-Unstable RFP (LVA-Tagged)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 619
    Illegal AgeI site found at 731
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick was synthesized by IDT. After linear pSB1C3 was amplified by PCR, the BioBrick was inserted into the backbone using NEB's HiFi Gibson assembly mix. The complete construct was transformed into both E.coli K12 DH5α and BL21. The plasmids were miniprepped and verified through sequencing.

Source

Promoter, RBS, LVA tag from E. coli
RFP from Discosoma striata

References