![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1613012:Design
Designed by: Ming-Cheng, Zhao. Chin-Fong,SU Group: iGEM15_HSNU-TAIPEI (2015-09-03)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 642
Illegal AgeI site found at 754 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream.
Source
Promoter, RBS, Terminator, and RFP are came from IGEM plate.