Device

Part:BBa_K1613012:Design

Designed by: Ming-Cheng, Zhao. Chin-Fong,SU   Group: iGEM15_HSNU-TAIPEI   (2015-09-03)


QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
    Illegal AgeI site found at 754
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is a QsrR Binding Site between promoter and RBS. Which can combine with Quinone-sensing and response Repressor(QsrR). And inhibit the transcription. While QsrR combines with BaP-1,6-Quinone, the QsrR will be activated and depart the Binding Site. That makes the transcription of downstream.

Source

Promoter, RBS, Terminator, and RFP are came from IGEM plate.

References

Ji Q, Zhang L, Jones MB, Sun F, Deng X, Liang H, et al. Molecular mechanism of quinone signaling mediated through S-quinonization of a YodB family repressor QsrR. Proceedings of the National Academy of Sciences. 2013 March 26;110(13):5010-5. (2013 IGEM CUHK)