Device

Part:BBa_K1613013:Design

Designed by: Ming-Cheng, Zhao CHIN-FONG,SU   Group: iGEM15_HSNU-TAIPEI   (2015-09-03)
Revision as of 03:02, 26 September 2015 by Poopooman (Talk | contribs) (→‎Design Notes)


Laccase and Quinone-sensing and response Repressor(QsrR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1570


Design Notes

We degrade benzo[a]pryene by Laccase. It will become bap-1,6-quinone. And bap-1,6-quinone can combine with QsrR which can repress K1613012. When bap-1,6-quinone combine with QsrR,it will be activated and go away.and K1613012 can work and produce rfp.

Source

From Igem plate.

References