Plasmid_Backbone
pSB4A5

Part:pSB4A5:Experience

Designed by: Reshma Shetty   Group: iGEM06_Bangalore   (2007-02-26)
Revision as of 21:39, 3 May 2007 by Rshetty (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB4A5

User Reviews

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pSB4A5
Reshma Shetty

When miniprepped, pSB4A5-I52001 does not give as high of yields as expected for a high copy plasmid likely due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin. I am working to address this issue. However, this plasmid should still work fine (it is just not as convenient to prep).

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