Generator

Part:BBa_K1602043

Designed by: Christian Sator,Stefan Zens, Max Zander, Benedikt Spannenkrebs, Sebastian Jaeger   Group: iGEM15_TU_Darmstadt   (2015-09-16)
Revision as of 05:47, 25 September 2015 by CSator (Talk | contribs)

araCpBad-hokD

Composite part consisting of an araC-regulated pBAD-promoter (BBa_K808000) cloned upstream of a ribosome binding site (BBa_B0034) and hokD (BBa_K1497008). Because of the araC-regulated pBAD-promoter production of the hokD is induced by the addition of arabniose which leads to cell death of E.coli. In the presence of glucose araCpBAD shows a very low basal expression level and therefore prevents the fatal expression of the toxin.

Functional Parameters

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None