Part:BBa_K1777003
miR-21 linear sponge
(β-globin SA)-(miR-21 binding site)6-(β-globin SD) | |
---|---|
Function | Linear miR-21 sponge |
Use in | Mammalian cells |
RFC standard | RFC 10 |
Backbone | pSB1C3 |
Submitted by | [http://2015.igem.org/Team:Fudan Fudan 2015] |
The Fudan iGEM team 2015 designed the part which has 6 miR-21 binding sites and is flanked by β-globing intron1 splicing site. We used it as the linear miR-21 sponge to sequester miR-21, compared with the blank control groups and circRNA sponge groups. In addition, adding cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.
As the controls of blank groups, the linear miR-21 sponges have the similiar constructs with "Ouroboros"(Part:BBa_K1777016) and only lack inverted repeat sequences.
Figure 1: Construct design.
Figure 2: Examination of efficiency of miR-21 sponges.
At an appropriate molar ratio of luciferase tools and sponges,the expression of Renilla shall increase.
Proof of function
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 81
Illegal XhoI site found at 452 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 423
None |