Part:BBa_K1616005

Holin/endolysin reversed - RBS and double T7 terminator

Holin - Endolysin

Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

Reverse sequence

This composite part is composed of the reverse sequence of Holin and the reverse sequence of Endolysin.

Fig. 1: Holin - Endolysin construction

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 306
  Illegal AgeI site found at 376
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.

Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (double terminator T7 reversed).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4