Part:BBa_K1616006

Endolysin reversed

Endolysin works with holin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)

This part is the reversed sequence of the endolysin; thus this part can be used with a reversed holin, reversed promoter, reversed RBS and reversed terminator.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 169
  Illegal AgeI site found at 239
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The illegal sites have been checked

Source

This sequence is the reverses sequence of the BBa_K112806 (Endolysin).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4