Composite
P(Cat)TetR

Part:BBa_K1846003:Design

Designed by: Luba Prout   Group: iGEM15_Birkbeck   (2015-08-28)
Revision as of 22:58, 20 September 2015 by LubaProut (Talk | contribs)

TetR circuit with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TetR generator cassette BBa_P0140 and P(Cat) promoter BBa_I14033 were obtained from 2015 iGEM distribution kit. and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis and Sanger sequencing. This BioBrick has been registered as part BBa_K1846003.


File:BBKiGEM-Pcat-TetR.jpg


Source

2015 iGEM distribution kit


References