Composite
P(Cat)TetR

Part:BBa_K1846003:Design

Designed by: Luba Prout   Group: iGEM15_Birkbeck   (2015-08-28)

TetR circuit with constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TetR generator cassette BBa_P0140 and P(Cat) promoter BBa_I14033 were obtained from 2015 iGEM distribution kit and cloned into a single pSB1C3 vector. Correct cloning of the parts into the pSB1C3 shipping vector was confirmed by agarose gel electrophoresis (Figure 1) and Sanger sequencing. This BioBrick has been registered as part BBa_K1846003.

BBKiGEM-Pcat-TetR.jpg


Source

2015 iGEM distribution kit


References