Protein_Domain

Part:BBa_K1486003:Experience

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-12)
Revision as of 07:44, 20 September 2015 by Xuhangli (Talk | contribs) (→‎Applications of BBa_K1486003)

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Applications of BBa_K1486003

Group: iGEM15_Fudan
Author: Xuhang Li
Summary:We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS.Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.

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UNIQ62b6b6ebc12b42e3-partinfo-00000000-QINU UNIQ62b6b6ebc12b42e3-partinfo-00000001-QINU