Part:BBa_K1486003:Experience

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User Reviews

UNIQb36ba9cd36843948-partinfo-00000000-QINU UNIQb36ba9cd36843948-partinfo-00000001-QINU UNIQb36ba9cd36843948-partinfo-00000002-QINU

•••• Fudan iGEM 2015

We developed a new part(BBa_K1777005) based on this parts.When we planned to use this part to link our fusion component,we found it hard to design a proper primer to amplify it because of mutiple primer binding site as a result of tandem GGGS. Therefore, we first improved it by change the first GGGS to a natural linker obtained from linker database.In addition,the codon is optimized for mammalian, and we insert a NLS to promote locating in cell nucleus. Such an improved GS linker could be easily assembled to your protein via overlap PCR and offer the property of nuclear localization.

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The Sao Carlos-Brazil Team has developed a yeast codon-optimized version of this part with size doubled, it can be accessed at https://parts.igem.org/Part:BBa_K3273015