Reporter

Part:BBa_J69511

Designed by: James Brown   Group: Haseloff Lab   (2009-04-06)
Revision as of 17:22, 19 September 2015 by Yinyuan1 1 (Talk | contribs)

GFP reporter device - LacI repressible

Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct.

1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases.

Our library entry can be found from BBa_K1676037 to BBa_K1676072.

Results of the mutant library:

Rbs-spots.jpeg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


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