Reporter

Part:BBa_J69511

Designed by: James Brown   Group: Haseloff Lab   (2009-04-06)

GFP reporter device - LacI repressible

Hi, we are from NTU-Singapore and we have made single basepair mutants of the RBS used in this reporter construct, BBa_B0034

1AT denotes substitution of A to T at the first base pair position, so on so forth. Based on BBa_B0034, we mutated all 12 base pairs of the RBS by changing each base with the other three bases. These mutants were then characterise in Shewanella oneidensis MR1.

Our library entry can be found from BBa_K1676037 to BBa_K1676072 where GFP expression monitoring data can be found. :)

MUTATIONS.png

Results of the mutant library:

Rbs-spots.jpeg

Sumary.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


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