Scar

Part:BBa_K1583015

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-08)
Revision as of 01:04, 19 September 2015 by Maxvthof (Talk | contribs)

Linker in fusion protein

A linker with the protein sequence GSGGSG was placed in between the CsgA (BBa_K1583000) and the affinity tags, His and Hydroxyapatite, for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.

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