![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1758320:Design
Designed by: Team Bielefeld-CeBiTec 2015 Group: iGEM15_Bielefeld-CeBiTec (2015-08-30)
Revision as of 21:49, 18 September 2015 by Gila (Talk | contribs) (Created page with "===Design Notes=== <html> We used the codon optimized, synthesized Sequence of cueR from <i> E.coli </i> under the control of a constitutive Primer (BBa:K608002). We designed t...")
Design Notes
We used the codon optimized, synthesized Sequence of cueR from E.coli under the control of a constitutive Primer (BBa:K608002). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and cueR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+ cueR and the pSB1C3:
cm_rev TATACGCAAGGCGACAAG
pSB1C3_kPrm_fwd CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA
pSB1C3_cueR_rev GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT
cm_fwd CGGCATCAGCACCTTGTC
Source
Synthesized, codon optimized gene of E. coli Synthesized by IDT
References
Synthesized by IDT