Composite

Part:BBa_K1758320:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-30)

Design Notes

We used the codon optimized, synthesized Sequence of cueR from E.coli under the control of a constitutive Primer (BBa:K608002). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the constitutive Promoter and cueR with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized constitutive promoter+ cueR and the pSB1C3:

cm_rev

TATACGCAAGGCGACAAG

pSB1C3_kPrm_fwd

CTAGGACTGAGCTAGCTGTCAATACTAGTAGCGGCCGCTGCA

pSB1C3_cueR_rev

GGTTGTTGTCATCATCGCGCAGGGTAACTCTAGAAGCGGCCGCGAAT

cm_fwd

CGGCATCAGCACCTTGTC

Source

Synthesized, codon optimized gene of E. coli Synthesized by IDT

References

Synthesized by IDT