Composite

Part:BBa_K1714004

Designed by: Masaya Mitsumoto   Group: iGEM15_HokkaidoU_Japan   (2015-09-13)
Revision as of 18:31, 18 September 2015 by Tamaro (Talk | contribs)

pBad-RBS B0034-BLA-double terminator

BBa_K1714001 (Plac-B0034-ABF-2-dT) will be finally introduced upstream of this parts and BLA expressed from this construct as Partner Proteins in Co-expression system.

Hokkaidou_co-expression_Fig.4.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1578
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None