Part:BBa_K1714004
pBad-RBS B0034-BLA-double terminator
BBa_K1714001 (Plac-B0034-ABF-2-dT) will be finally introduced upstream of this parts and BLA expressed from this construct as Partner Proteins in Co-expression system.
We choose four Lactalbumin family proteins, concretely HLA (Human α-Lactalbumin), BLA (Bovine α-Lactalbumin), HLZ (Human Lysozyme), BLZ (Bovine Lysozyme), as partner proteins. Because these proteins have negative charge and ABF-2 have positive charge like thanatin, they will interact and form inclusion bodies. There is study which used these proteins for formation of inclusion bodies with ABF-2. (2013. S. Tomisawa et al.)
Because, as referred to above, thanatin also has positive charge, it is projected that thanatin interacts Lactalbumin family proteins which have negative charge. For reference, the isoelectric point (pI) of ABF-2 is 9.1, on the other hand, pI of thanatin is 10.47. On the other hand, pI of HLA is 4.7, BLA is 4.8, HLZ is 9.3 and BLZ is 6.5. We think the value of pI of thanatin is enough similar with ABF-2 to apply this method for ABF-2 to thanatin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1578
Illegal SapI site found at 961
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