Generator

Part:BBa_K1758311:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-30)
Revision as of 16:37, 18 September 2015 by Luzia (Talk | contribs)

_NOTOC__ Fumarate reductase (frd) from E. coli under the control of the T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 666
    Illegal AgeI site found at 741
    Illegal AgeI site found at 3023
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

===Source

The original source of our chromate responsive promoter is Ochrobactrum tritici 5bvl1. We used a codon optimized version which we got synthesised by IDT.

References