Generator

Part:BBa_K1758311:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-30)

mRFP under control of the chromium responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the codon optimized, synthesized Sequence of RFP under the control of chrP from iGEM Team BIT 2013( BBa_K346002 ). We designed the used primes, in specially split primers, for Gibson Assembly to ligate the RFP under control of chrP with pSB1C3. For this aim we designed four primers for the generation of homologous overlaps between the synthesized RFP under control of chrP and the pSB1C3:

cm_fwd (CGGCATCAGCACCTTGTC)

pSB1C3_chrP_rev (CAAATGGCAATAGGAATAAGCAATCCTCTAGAAGCGGCCGCGAAT)

cm_rev (TATACGCAAGGCGACAAG)

pSB1C3_RFP_rev (CCGCCACTCAACGGGTGCCTGATAATACTAGTAGCGGCCGCTGCA)

Source

Synthesized, codon optimized gene of Ochrobactrum tritici synthesized by IDT

References

Synthesized by IDT

http://www.ncbi.nlm.nih.gov/nuccore/134290393