DNA

Part:BBa_K1789005:Design

Designed by: Xinyuan Qiu   Group: iGEM15_NUDT_CHINA   (2015-09-14)
Revision as of 11:07, 18 September 2015 by Qiuxinyuan12 (Talk | contribs) (→‎Design Notes)


SCAF1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
    Illegal AgeI site found at 38
    Illegal AgeI site found at 68
    Illegal AgeI site found at 98
    Illegal AgeI site found at 128
    Illegal AgeI site found at 158
    Illegal AgeI site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.

This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs

Source

This part is synthesized by company

References