Part:BBa_K1789005:Design

SCAF1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 8
  Illegal AgeI site found at 38
  Illegal AgeI site found at 68
  Illegal AgeI site found at 98
  Illegal AgeI site found at 128
  Illegal AgeI site found at 158
  Illegal AgeI site found at 188
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The length of DNA BMs and intervening sequences is designed based on the typical in vivo B-type DNA structure, and the Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors.

This scaffold is designed to put enzymes as close as possible, no intervening sequence is inserted between two BMs.

Source

This part is synthesized by company