Reporter
ECFP

Part:BBa_E0022

Designed by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman   Group: Antiquity   (2003-01-31)
Revision as of 23:59, 17 September 2015 by SylvieL (Talk | contribs) (→‎Usage and Biology)


enhanced cyan fluorescent protein derived from A. victoria GFP

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.


Usage and Biology

This part can be used to test promoter strength or the proper functioning of a regulator device. The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.

In this experiment, the E0022 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728

Paris Saclay K1707028 w1POL.png Differential interference contrast

Paris Saclay K1707028 w2Lumencor CFP 434-17.png Excitation with led 437 nm/17nm (CFP), 100 ms of exposure

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
abs
biology
emissionCFP
emit476
excitation
excite439
lum
proteinECFP-LVA
tagLVA