Composite

Part:BBa_K1678006:Design

Designed by: Prateek Garg   Group: iGEM15_Paris_Bettencourt   (2015-09-16)
Revision as of 23:33, 16 September 2015 by Registry (Talk | contribs)

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mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883


Design Notes

None


Source

The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006 The terminator comes from the following publication: 'Spath et al. Microbial Cell Factories 2012, 11:141' The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150' The whole cassette was artificially synthesized.

References