Composite

Part:BBa_K1678006:Design

Designed by: Prateek Garg   Group: iGEM15_Paris_Bettencourt   (2015-09-16)


mCerulean gene cassette with P48 promoter and TldH terminator for lactobacillus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 883


Design Notes

The promoter comes from the following publication: 'A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum', Microbiology, 2006.
The Tldh terminator comes from L. buchneri lactate dehydrogenase gene. (also used in :'Spath et al. Microbial Cell Factories 2012, 11:141').
The RBS comes from the following publication: 'Tauer et al. Microbial Cell Factories 2014, 13:150'.
The whole cassette was artificially synthesized.

Source

References