Device
Part:BBa_K1819006
Designed by: Laís Ribovski Group: iGEM15_Brasil-USP (2015-09-16)
Constitutive promoter (J23106) and GFP (S05308)
Constitutive promotor from Anderson library (BBa_J23106) assembled to green fluorescent protein attached to linker (BBa_S05308). This part was used to characterize BBa_K1819000 and compare its efficiency to green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (BBa_E0040).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 717
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
//function/reporter
//function/reporter/fluorescence
color | Green |
direction | Forward |
emission | |
emit | 511 |
excitation | |
excite | 501 |
kegg | |
lum | |
protein | GFPmut3b |
swisspro |