Composite

Part:BBa_K1659501

Designed by: Wei Chung Kong   Group: iGEM15_Oxford   (2015-08-28)
Revision as of 04:28, 15 September 2015 by Weikongquee (Talk | contribs)

pLsr + RBS + sfGFP


This composite part consists of the AI-2 quorum sensor-inducible promoter BBa_K117002, a weak ribosome binding site BBa_B0031, and a GFP coding sequence BBa_I746916.


Biology

The AI-2 quorum sensing pathway is non-species specific cell-to-cell communication pathway used by both Gram-positive and Gram-negative bacteria and is found in at least 55 strains of them [1][2].



References

[1] Surette, M.G., Miller, M.B. & Bassler, B.L., 1999. Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production. Proceedings of the National Academy of Sciences of the United States of America, 96(4), pp.1639–1644.

[2] Bassler, B.L., Greenberg, E.P. & Stevens, A.M., 1997. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. Journal of Bacteriology, 179(12), pp.4043–4045.

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