Project
WOBS

Part:BBa_J45700:Design

Designed by: MIT iGEM 2006   Group: iGEM2006_MIT   (2006-10-30)
Revision as of 05:12, 31 October 2006 by Jasonk (Talk | contribs)

Wintergreen odor biosynthetic system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1689
    Illegal NgoMIV site found at 2093
    Illegal NgoMIV site found at 2218
    Illegal NgoMIV site found at 2229
    Illegal NgoMIV site found at 2510
    Illegal NgoMIV site found at 2978
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 285


Design Notes

We recommend use of strain BBa_J45999 as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.


Source

This system is composed of devices BBa_J45120 and BBa_J45320.

References