Part:BBa_K1351031

LMU Bacillus RBS collection 4

This part was generated in RFC10, and has the following prefix and suffix:

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aggagaggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI 

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number	BBa_K1310 ..	Sequence
1	28	aaggagggata
2	29	agaggaggata
3	30	aaggagagata
4	31	aggagaggata
5	32	aagaggagata
6	33	agaaagggata
7	34	aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]