RBS

Part:BBa_K1351031

Designed by: Florian Riemer   Group: iGEM14_LMU-Munich   (2014-10-06)

LMU Bacillus RBS collection 4

This part was generated in RFC10, and has the following prefix and suffix: 

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G aggagaggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number BBa_K1310 .. Sequence
1 28 aaggagggata
2 29 agaggaggata
3 30 aaggagagata
4 31 aggagaggata
5 32 aagaggagata
6 33 agaaagggata
7 34 aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//rbs/prokaryote/constitutive
Parameters
None