Generator
SAG

Part:BBa_J45300

Designed by: MIT IGEM 2006   Group: iGEM2006_MIT   (2006-08-21)
Revision as of 05:01, 31 October 2006 by Drephd (Talk | contribs)

Salicylic acid generator

This part is used to generate salicylic acid. Consists of promoter, RBS, pchBA, terminator. This part is almost identical to BBa_J45320, except that it uses the B0030 RBS instead of B0032 (which is in BBa_J45320). J45300.jpg

Salicylic Acid Generating Device (SAGD):

  • converts chorismate into salicylic acid
  • production of salicylic acid was verified by controlled tests: E. coli with WGD but no SAGD did not produce methyl salicylate (that is, they did not smell minty), while E. coli containing both the SAGD and WGD did produce methyl salicylate autonomously.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 920
    Illegal NgoMIV site found at 931
    Illegal NgoMIV site found at 1212
    Illegal NgoMIV site found at 1680
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None