![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
AgaA
Part:BBa_K1403002:Design
Designed by: Cristina Garcia-Timermans Group: iGEM14_Paris_Bettencourt (2014-10-06)
AgaA expression (3M2H-> 3H3MH)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 288
Illegal BamHI site found at 519 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization. Check no restriction sites for EcoRI, SpeI, XbaI and PstI.
Source
agaA sequence codon optimized for E. coli 12 (IDT online tool)