Coding
AgaA

Part:BBa_K1403002:Design

Designed by: Cristina Garcia-Timermans   Group: iGEM14_Paris_Bettencourt   (2014-10-06)

AgaA expression (3M2H-> 3H3MH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 288
    Illegal BamHI site found at 519
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized using [http://eu.idtdna.com/CodonOpt IDT online tool]. We checked that there were no restriction sites for EcoRI, SpeI, XbaI and PstI using the software Geneious.

Source

AgaA sequence ([http://www.ncbi.nlm.nih.gov/nuccore/AF534871.1 Genbank: AF534871.1]) codon optimized for E. coli 12 (IDT online tool)

References

  • [http://ncbi.nlm.nih.gov/pubmed/12468539 "A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla." Acuna G. et al ,2003]