Signalling

Part:BBa_J37015:Design

Designed by: Tom Hinson   Group: iGEM06_Imperial   (2006-07-17)
Revision as of 14:29, 30 October 2006 by Farah (Talk | contribs) (→‎Design Notes)

Prey Molecule Generator (AHL) + GFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2400


Design Notes

  • The part is designed to generate AHL via a positive feedback loop.
  • LuxR is constitutively produced by tetR, with LuxI under the control of Lux pR
  • The part has been ligated from the following previous parts: BBa_F2620,

BBa_C0261, BBa_I13504

  • Expression of GFP downstream of the promoter serves as an indicator of the production of LuxI.


  • More details about design and motivation behind the part can be found [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Design here].

Source

N/A

References

  • Part F2620 has previously been studied by Barry Canton. More information about this receiver can be found [http://openwetware.org/wiki/Endy:F2620 here].