Part:BBa_J37015:Design

Prey Molecule Generator (AHL) + GFP Reporter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1724
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1004
  Illegal BsaI.rc site found at 2400
  

Design Notes

• The part is designed to generate AHL via a positive feedback loop.
• LuxR is constitutively produced by tetR, with LuxI under the control of Lux pR
• The part has been ligated from the following previous parts: BBa_F2620,

BBa_C0261, BBa_I13504

• Expression of GFP downstream of the promoter serves as an indicator of the production of LuxI.

• [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Design MORE DETAILS about Design and Motivation behind the part can be found here].

Source

N/A

References

• Part F2620 has previously been studied by Barry Canton. More information about this receiver can be found [http://openwetware.org/wiki/Endy:F2620 here].