Part:BBa_R0080

Promoter (AraC regulated)

AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC or the simple sugar [http://openwetware.org/wiki/Arabinose arabinose]

b/c the operator lacks the araO2 site, there should not be araC-mediated repression.

Usage and Biology

araC binding region from E.coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 103
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution: iGEM14_NUDT_CHINA

Group: iGEM14_NUDT_CHINA/ Author: Xinyuan Qiu

Summary: We tested expression of this part in E.coli, and corrected the founction description of this part.

Methods and Practices

We used the circuit of BBa_K1532008 to test the function of this part, the sequence and features of the part BBa_K1532008 is as follows:

Fluorometric measurement were processed with Fluoroskan(R) Ascent FL from ThermoTM using the filters of 485nm and 538nm. The E.coli to be tested was cultured in liquid LB media containing 35μg/mL chloramphenicol to OD1.6, than split into 96 well plates (from ThermoTM) by 150μL/well. The plate was then put into the Fluoroskan(R) Ascent FL and cultured in 25℃ and background shake of 90rpms. The Fluorometric measurement was processed with the interval time of 10 minutes.

The layout of the plate is as follows: