Coding

Part:BBa_K1316006

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-09-12)
Revision as of 21:38, 17 October 2014 by Joanctd.igem (Talk | contribs)

Rhamnose promoter coupled to N-genes

nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism because when these genes were knocked out, the response of the yqjF and ybiJ promoters decreased 1,000 times.[1] This construct was made so that these genes are regulated by the inducible Rhamnose promoter


Characterisation

According to our results, these N-genes play an important role in the activation of ybiJ and yqjF promoters in presence of 2,4-DNT. For more information, visit the <a href="http://2014.igem.org/Team:TU_Delft-Leiden/WetLab/landmine/characterisation"> Landmine Detection module characterisation </a> on our wiki page or the pages for constructs BBa_K1316007 and BBa_K1316008 !

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References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None