Part:BBa_K1316006

Rhamnose promoter coupled to N-genes

nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism because when these genes were knocked out, the response of the yqjF and ybiJ promoters decreased 1,000 times.[1] This construct was made so that these genes are regulated by the inducible Rhamnose promoter

Characterisation

According to our results, these N-genes play an important role in the activation of ybiJ and yqjF promoters in presence of 2,4-DNT. For more information, visit the Landmine Detection module characterisation on our wiki page or the pages for constructs BBa_K1316007 and BBa_K1316008 !

References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]