Part:BBa_K1351039

P_xylA, a xylose inducible promoter for Bacillus subtilis

P_xylA is the native promoter of the Bacillus subtilis xylose degradation locus xyl. The promoter is regulated by the repressor XylR which is not encoded by this BioBrick. The regulation of P_xylA is only ensured, if you are working with B. subtilis as a chassis.

The BioBrick was evaluated with the integrative vector pBS3Clux, so the promoter activity was measured via luminescence output in B. subtilis.

The data (fig.1) clearly shows that P_xylA can be gradually induced about 100-fold over background.

Fig. 1: Promoter activity of PxylA depending on the xylose concentration (0% to 0.2%). Panels A and D show cell density, B and E luminescence intensity (normalized by cell density). Panels C and F show dose-response curves one hour post-induction. Data derive from three independent experiments.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 247
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]