Reporter

Part:BBa_K1541009

Designed by: Daniel Gerngross   Group: iGEM14_ETH_Zurich   (2014-10-06)
Revision as of 20:19, 17 October 2014 by Danger (Talk | contribs)

sfGFP under promoter P(Rhl) with riboregulator RR12

ETH Zurich 2014 LuxWith and WORibo.png
ETH Zurich 2014 LuxWith and WOSites.png

this riboregulated promoter construct contains the quorum sensing promoter BBa_I14017 which can be activated in presence of RhlR (BBa_C0171) and C4-HSL, the product of the enzyme RhlI (BBa_C0170), succeeded by the gene for sfGFP. However, the promoter BBa_I14017 by itself shows some leakiness. Together with the ribogegulator 12[18] the leakiness could be reduced.

ETH Zurich 2014 RhlWith and WORibo.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 765
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None